Your ultimate goal is to safeguard the surviving and united population of the settlements. 7 months after the spreading of the virus you have taken on the role of Division agent trying to save the remaining humanity from this deadly virus. Genome sequencing Next-generation sequencing platforms RNA viruses.Description:Tom Clancys The Division 2 is an advanced series of previous versions that tell about adventures in Washington city that have been infected with deadly virus.Tom Clancys The Division 2 is housed in the most protected city on Earth, Washington DC. These findings suggest that the Ion Torrent S5 and Illumina MiSeq platforms are both viable options for genomic sequencing of RNA viruses, each with specific advantages and tradeoffs. For lower throughput sequencing runs (i.e., Ion Torrent 510 and Illumina MiSeq Nano V2), the cost per sample was lower on the MiSeq platform, whereas with higher throughput runs (Ion Torrent 530 and Illumina MiSeq V2) there is less of a difference in the cost per sample between the two sequencing platforms ($5.47-$10.25 more per sample for an Ion Torrent 530 chip run when multiplexing 24 samples).
However, indels at homopolymer regions impacted the accuracy of consensus genome sequences. The Ion Torrent S5 510 chip runs produced more reads at a lower cost per sample than the highest output Ion Torrent PGM 318 chip run, and generated the highest proportion of reads for enterovirus D68 samples. The choice of library preparation kit and sequencing platform was found to impact the breadth of genome coverage and accuracy of consensus viral genomes. The specimens were processed, using combinations of three library preparation and five sequencing kits, to assess the quality and completeness of assembled viral genomes, and an estimation of cost per sample to generate the data was calculated.
The Illumina MiSeq, Ion Torrent PGM and Ion Torrent S5 platforms were evaluated using a panel of sixteen specimens containing picornaviruses and human caliciviruses (noroviruses and sapoviruses). While Illumina® and Ion Torrent® sequencing platforms are used extensively for generating viral RNA genome sequences, there is limited data comparing different platforms.
Next-generation sequencing is a powerful tool for virological surveillance.
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